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2012, Volume 28, Number 3, Page(s) 238-244     
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DOI: 10.5146/tjpath.2012.01130
Immunohistochemical Characteristics of Triple Negative/Basal-like Breast Cancer
Emel Ebru PALA, Ümit BAYOL, Süheyla CUMURCU, Elif KESKİN
İzmir Tepecik Eğitim Araştırma Hastanesi, Patoloji Bölümü, İzmİr, TÜRKİYE

Department of Pathology, İzmir Tepecik Education and Research Hospital, İZMİR, TURKEY

Keywords: Breast, Carcinoma, Immunohistochemistry

Objective: Triple-negative-breast-cancer that accounts for 10-20% of all breast carcinomas is defined by the lack of estrogen receptor, progesterone receptor, HER2 expression, and agressive clinical behavior. Triple-negative-breast-cancer is categorized into basal like and other types. The basal-like subtype is characterized by the expression of myoepithelial/basal markers.

Material and Method: We studied 41 immunohistochemically triplenegative- breast-cancer patients to determine EGFR, Cytokeratine 5/6, p53, Ki67, GCDFP-15 expression profiles, HER2 and Chromosome 17 centromere gene status by fluorescence-in-situ-hybridization method.

Results: Histological type was invasive ductal carcinoma in 90.2% of the tumors. p53, Ki67, GCDFP-15 mean positivity rates were 55.6%, 51.7%, and 3.2%, respectively. GCDFP-15 positivity was noted in 8 cases of which 6 were Cytokeratine 5/6 negative. The cut-off value for Cytokeratine 5/6 positivity was 5%. EGFR immunoreactivity was grouped into 0, 1+ as negative; 2+, 3+ as positive categories. Cytokeratine 5/6 was positive in 56,1%, EGFR was positive in 51.2% of the patients. The relation between Cytokeratine 5/6 and EGFR expression was statistically significant (p<0.01). None of the cases showed HER2 amplification by fluorescence-in-situ-hybridization method.

Conclusion: GCDFP-15 alone is not a useful marker to detect the metastasis of basaloid type breast cancers. Cytokeratine 5/6 and EGFR expressions showed correlation so these markers are reliable to diagnose basaloid type tumors with a 5% cut-off value.

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