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2018, Volume 34, Number 1, Page(s) 019-028
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DOI: 10.5146/tjpath.2017.01409 |
Immunohistochemical Analysis of the Extrinsic Apoptosis Process in the Non-Neoplastic and Neoplastic Prostate |
Ayşe Burcu İLERİ1, Reşit Doğan KÖSEOĞLU1, Fatma MARKOÇ1, İlker ETİKAN2, Doğan ATILGAN3, Faik Alev DERESOY1 |
1Department of Pathology, Gaziosmanpasa University School of Medicine, TOKAT, Turkey 2Department of Biostatistics, Near East University School of Medicine, Lefkoşa, North Cyprus , Mersin 10, Turkey 3Department of Urology, Gaziosmanpasa University School of Medicine, TOKAT, Turkey |
Keywords:
Prostate, Carcinogenesis, Fas, FasL, DcR1, FLIP |
Objective: Deviations in the apoptotic process have been demonstrated in prostate carcinogenesis. We aimed to evaluate especially the process of
extrinsic apoptosis in the spectrum of neoplastic lesions of the prostate epithelium so as to reveal the variations in the apoptotic process.
Material and Method: The study included 20 benign prostatic hyperplasia, 8 high-grade prostatic intraepithelial neoplasia and 82 prostatic
carcinoma patients. Immunohistochemistry was performed on sections obtained from materials of suprapubic prostatectomy, tru-cut biopsy,
transurethral resection and radical prostatectomy. While Fas and FasL were evaluated in glandular and stromal areas, DcR1 and FLIP were
evaluated in only glandular areas. Intensity and extent of immunostaining for Fas and FasL antibodies were separately scored and both scores
were summarized. The total score of ≥ 4 both for Fas and FasL, expressions of FLIP and DcR1determined in more than 5% of glandular areas
were accepted as positive.
Results: Glandular FasL positivity was observed in 63.8 and 20% of the cases with prostatic carcinoma and benign prostatic hyperplasia,
respectively (p=0.001). The loss of stromal Fas expression in PCa was obvious (p<0.001). FLIP positivity was more frequently seen in high-grade
prostatic intraepithelial neoplasia and PCa.
Conclusion: In prostatic carcinoma, decreased stromal Fas expression, contrary to higher glandular FasL positivity, supports the assertion
that sensitivity of epithelial and stromal cells to apoptosis and their protective pathways against apoptosis undergo alterations. Increased FLIP
expressions in high-grade prostatic intraepithelial neoplasia and prostatic carcinoma can also be interpreted accordingly.
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