Turkish Journal of Pathology - 2005; 21(3-4)

PROF. DR. ABD�LSETDAR �ZT�RK’�N ARDINDAN

[S�tk� |TUZLALI|1] — 2004-12-01

p53, bcl-2, BAX EXPRESSION IN BASAL CELL CARCINOMAS AND NONTUMORAL SURROUNDING SKIN

Sema B�RCAN, �zden �ANDIR, Nilg�n KAPUCUO�LU, �irin BA�PINAR — 2004-12-01

The aim of this study is to investigate p53, bcl-2 and bax expressions in basal cell carcinoma (BCC) and nontumoral surrounding epidermis. p53, bcl-2 and bax expression was investigated by streptavidin-biotin-peroxidase technique in 54 solid type BCCs, and immunoreactivity was evaluated as negative (-), mild (+1), moderate (+2) and marked positive (+3). The mean age of the patients was 62 years. Forty two tumors were located on the head neck, 3 on the extremities and 1 on the trunk. Fourty one and 13 BCCs were solid nodular and infitrative types, respectively. In the nontumoral epidermis, p53 positivity was detected in 66.7% of the cases. While basal cells and hair follicles were positive with bcl-2, heterogeneous suprabasal bax positivity was observed in surrounding nontumoral epidermis. In BCCs, p53 was positive in 41 (75.9%) of the cases. There was a statistically positive correlation between nontumoral epidermis and BCC in relation to p53 immunoreactivity (p=0.004, r=0.392). In addition, the level of p53 positivity in the BCCs having p53 positive nontumoral epidermis was statistically higher than the negative cases (p=0.034). With bcl-2, usually homogeneous high levels of bcl-2 positivity were found in 40 (74.5%) cases, and bax showed heterogeneous positivity in 24 (43.6%) BCCs. There was a significant positive correlation between bcl-2 and bax expression (p=0.039, r=0.282). Considering tumor growth pattern, bcl-2 was higher in solid nodular BCCs than the infiltrative types (p=0.002. According to our findings, the presence of high percentage of p53 positivity in both nontumoral epidermis and BCC may show the association between p53 and chronic sun damage and the importance of UV in the etiopathogenesis of BCC. High level of bcl-2 expression in BCCs is decreased in the infiltrative type BCC. Although these findings show that bax protein is involved in apoptosis regulation and it has a positive correlation with bcl-2 in the BCCs, there was no association with tumor growth pattern.

ASSOCIATION OF MACROPHAGES, EOSINOPHIL LEUKOCYTES AND NK CELLS WITH ANGIOGENESIS AND DEPTH OF INVASION IN COLORECTAL CARCINOMAS

2004-12-01

Colorectal carcinoma is one of the most frequently observed malignancy in the developed countries. Angiogenesis is essential for tumor growth and metastasis. The associations between peritumoral inflammatory cells and natural killer cells and angiogenesis to depth of invasion and lymph node metastasis were investigated in 75 colorectal adenocarcinoma specimens. There was not any statistically significant correlation between depth of invasion, lymph node metastasis and microvessel density, peritumoral macrophages, eosinophil leucocytes and naturel killer cells. There was a positive correlation between angiogenesis and peritumoral macrophages (p=0.021). Also natural killer cells and eosinophil leucocytes have a positive correlation (p=0.027). The relation between angiogenesis and eosinophil leucocytes and NK cells was insignificant. These data indicate that peritumoral inflammatory cells may contribute to tumor angiogenesis, and the sinergistic effect between peritumoral inflammatory cells.

PSEUDOANGIOMATOUS STROMAL HYPERPLASIA OF THE BREAST (MORPHOLOGICAL AND IMMUNOHISTOCHEMICAL ANALYSIS OF 14 CASES)

2004-12-01

Pseudoangiomatous stromal hyperplasia (PASH) is an uncommon, benign mesenchymal lesion of the breast. Histologically, it is characterized by complex, anastomosing, empty, slit-like spaces that are lined by spindle cells in a dense collagenous stroma. We have evaluated 14 PASH cases morphologically and immunohistochemically, using antibodies against CD34 and progesterone receptor and reviewed the relevant literature. Also, we have evaluated the mast cell population of the lesions, using Giemsa stain. All of the cases were women presenting with a mass in the breast. The diameter of the lesions ranged from 1 cm to 13,5 cm (mean diameter 4 cm). The spindle cells, lining the slit-like spaces, were immunoreactive for anti CD34 whereas all were negative for anti progesterone receptor. The mean mast cell count was 17,5/10 HPF. Although differential diagnosis of PASH includes several mesenchymal lesions appropriate macroscopic sampling and immunohistochemistry are usually sufficient for proper diagnosis. Nevertheless, detection of immunostaining for CD34, using immunohistochemistry, may support the morphologic diagnosis.

REPRODUCTIBILITY OF THE ISHAK MODIFIED HISTOLOGIC ACTIVITY INDEX IN THE EVALUATION OF CHRONIC VIRAL HEPATITIS

O�uz AYDIN, Levent YILDIZ, Mehmet KEFEL�, Sancar BARI�, Bedri KANDEM�R — 2004-12-01

Biopsies from 55 serologically confirmed chronic hepatitis B(30 ), C(22) and, B and C (3) cases were independently evaluated by two pathologists and Ishak modified histologic activity indices were determined. Study was repeated two months later. Both interobserver and intraobserver reproducibility were assessed using kappa statistics. Interobserver reproducibility of the “periportal or periseptal interface hepatitis (piecemeal necrosis)” and “focal lytic necrosis, apoptosis and focal inflammation” parameters forming Ishak modified histologic activity index was found to be low. It is concluded that Ishak modified histologic activity index, especially these parameters, should be revised to increase reproducibility.

THE UTILITY OF HISTOMORPHOLOGICAL FEATURES IN THE DIFFERENTIATION OF EOSINOPHILIC CHROMOPHOBE RENAL CELL CARCINOMA AND ONCOCYTOMA

�zg�r METE, I��n KILI�ASLAN, Veli UYSAL — 2004-12-01

Renal oncocytomas and eosinophilic variants of chromophobe renal cell carcinomas have similar histological and cytological features. In order to identify some histological parameters for differentiating these tumours, 51 renal oncocytomas and 32 chromophobe renal cell carcinoma were included in our study. Nuclear, cytoplasmic, structural and stromal features are investigated in both tumours. The presence of well-defined nuclear border, fibrohyalinised scar, stromal oedema and entrapped tubules are found to be significant (p<0,05) for oncocytomas. The presence of necrosis, perinuclear halo and special vascular network are found to be significant (p<0,05) in chromophobe renal cell carcinomas.

EPIGLOTTIC SARCOMATOID CARCINOMA WITH EXTENSIVE RHABDOMYOSARCOMATOUS AND OSTEOSARCOMATOUS FEATURES: A CASE REPORT

Kemal DEN�Z, Sedat �A�LI, Olgun KONTA� — 2004-12-01

A case of sarcomatoid carcinoma in a 75-year-old male with epiglottic location was presented. Histological examination of the tumor revealed rhabdomyosarcoma and osteosarcoma-like areas admixed with a minor component of squamous cell carcinoma. The rhabdomyosarcomatous component showed strong vimentin, desmin and myoglobin immunoreactivity. Histologically most of these tumors consisted of conventional squamous cell carcinoma and sarcomatoid component which frequently arranged in a storiform pattern. A combination of extensive osteosarcomatous and rhabdomyosarcomatous component has been reported in rare cases. A diagnosis of laryngeal sarcomatoid carcinoma requires considerable caution when the sarcomatous portion predominates and care must be taken not to overlook the carcinomatous areas.

ENDOMETRIOSIS IN ACCESSORY OVARY: A CASE REPORT

Yurdanur S�LL�, Gamze D�NMEZ, Bedri KANDEM�R — 2004-12-01

Accessory ovary, described as extra ovary either adjacent or connected to normal ovaries, is a rare gynecological anomaly. Accessory ovaries may develop secondary to embryogenetic abnormalities, or to damage of normal ovaries due to inflammation or surgery. It is usually asymptomatic and defined incidentally. We report a rare case with endometriosis in accessory ovary, which was operated with diagnosis of ovarian cyst and myoma uteri.