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2010, Volume 26, Number 3, Page(s) 216-221     
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DOI: 10.5146/tjpath.2010.01027
Evaluation of Immunohistochemistry and Silver-Enhanced In Situ Hybridization Results for HER2/neu Manually and with Image Analysis System in Human Breast Cancer
Doğuş ÖZDEMİR, Işın PAK
Department of Pathology, Abdurrahman Yurtarslan Ankara Oncology Hospital, ANKARA, TURKEY
Keywords: Immunohistochemistry, In situ hybridization, Computerassisted image analysis, Breast cancer, HER2/neu

Objective: HER2/neu (ErbB2) gene status is one of the important information while planing terapy in breast carcinoma. For HER2/ neu testing there is not standart assay that has been agreed on. Silver enhanced in situ hybridization is a cantitative and highly reproducible assay. Immunohistochemistry is a cheap and easy assay that has disadvantage of being less reproducible. Recently developed pathologist assisted computerized image analysis systems decrease the ratio of subjectivity due to manual evaluation, enable tele-consultation and make it easy to evaluate tumor morphology and markers. Our aim is to investigate the consistency of manual and computerized interpretation of the results of immunohistochemistry and silver enhanced in situ hybridization.

Material and Method: Immunohistochemisty and silver enhanced in situ hybridization of 73 invasive breast carcinoma results were evaluated manually to determine HER2/neu status. Later, silver enhanced in situ hybridization and immunohistochemistry results were reevaluated with Ventana Image Analysis System. Afterwards correlation of both methods with image analysis system and manuel interpretation were calculated.

Result: All cases were score 2 with immunohistochemistry. With image analysis system, 5 cases were score 1, 56 cases were score 2 and 12 cases were score 3. When in situ hybridization results were reevaluated with image analysis system, 6 cases were discordant compared with manual interpretation.

Conclusion: The correlation rate of immunohistochemistry interpretation results between manuel method and image analysis system was %76; but silver enhanced in situ hybridization interpretation results between manuel method and image analysis system were %91 concordant and it was statistically significant (k= 0.832 and p<0.001).


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