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2022, Volume 38, Number 3, Page(s) 213-218
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DOI: 10.5146/tjpath.2021.01565 |
Methylation Profiling of Specific Genes in Ependymomas |
Naz KANIT1, Pelin YALCIN2, Serhat ERBAYRAKTAR3, Erdener OZER4 |
1Department of Molecular Medicine, Dokuz Eylul University Institute of Health Sciences, IZMIR, TURKEY 2Department of Medical Biology, Dokuz Eylul University Institute of Health Sciences, IZMIR, TURKEY 3Department of Neurosurgery, Dokuz Eylul University School of Medicine, IZMIR, TURKEY 4Department of Pathology, Dokuz Eylul University School of Medicine, IZMIR, TURKEY |
Keywords:
DNA methylation, Ependymoma, Epigenetics, Pyrosequencing |
Objective: Ependymomas are neuroepithelial tumors of the central nervous system with heterogeneous biology and clinical course. The aim of
the present study is to investigate the relationship between the methylation status and clinicopathological parameters in ependymomas.
Material and Method: DNA methylation status of CDKN2A, RASSF1A, KLF4 and ZIC2 genes were quantitatively analyzed with pyrosequencing
in 44 ependymoma tumor tissues. The relationship of methylation profiles with tumor subtype, histological grade and patient age was statistically
analyzed.
Results: DNA methylation analyses for CDKN2A revealed no difference in methylation levels. Of the 31 included samples for optimal ZIC2
methylation analysis, 10 were hypermethylated (32.3%) and this change was significantly found in the adult spinal ependymomas (p=0.01).
KLF4 hypermethylation was observed in 6 of the overall included 35 samples (17.1%); however, there was no statistically significant relation of
the methylation status with tumor subtype, histological grade or age group. RASSF1A hypermethylation was observed in overall 40 included
samples with varying methylation levels. Higher levels of hypermethylation were significantly related to the grade 3 histology (p=0.01) and
spinal ependymomas (p=0.006). The pediatric cases with grade 3 ependymomas and ependymomas of adulthood showed significantly increased
RASSF1A hypermethylation levels (p<0.001 and p=0.001, respectively).
Conclusion: DNA methylation changes are likely to have biological importance in ependymomas. Both ZIC2 and RASSF1A methylation status
may be useful parameters in the subclassification of these tumors.
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