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2022, Volume 38, Number 2, Page(s) 083-089
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DOI: 10.5146/tjpath.2022.01568 |
Role of Immunohistochemistry in the Differential Diagnosis of Pediatric Renal Tumors: Expression of Cyclin D1, Beta-Catenin , PDGFR-Alpha, and PTEN |
Nuray KEPIL1, Şebnem BATUR1, Zeynep Ecem KAIN1, Gamze ÖZCAN1, Şenol EMRE2, Rahşan ÖZCAN2, Tülin Tiraje CELKAN3, Nil ÇOMUNOĞLU1 |
1Department of Pathology, Istanbul University, Cerrahpasa Faculty of Medicine, ISTANBUL, TURKEY
2Department of Pediatric Surgery, Istanbul University, Cerrahpasa Faculty of Medicine, ISTANBUL, TURKEY
3Department of Pediatrics Oncology, Liv Hospital, ISTANBUL, TURKEY |
Keywords:
Cyclin D1, Immunohistochemistry, Pediatric pathology, Renal tumors, Wilms tumor |
Objective: Pediatric renal tumors overlap histomorphologically and may cause misdiagnosis. We aimed to determine the role of
immunohistochemical staining of Cyclin D1, PTEN, beta-catenin and PDGFR-alpha on pediatric renal tumors.
Material and Method: Thirty-six cases of 8 different tumors were included in the study. Four blocks of paraffin tissue microarray were
constructed. Cyclin D1, PTEN, beta-catenin and PDGFR-alpha were used in all cases. Staining intensity and extent were graded.
Results: All cases of clear cell sarcoma (CCS) and epithelial components of Wilms tumor (WT) showed immunopositivity for Cyclin D1 but
blastemal and stromal components of WT were negative. All cases of CCS and most cases of WT consisting of blastemal and stromal components
demonstrated loss of expression with PTEN.
Conclusion: Cyclin D1 is not a specific immunohistochemical marker due to its strong and diffuse positivity in CCS cases. It may be useful to
differentiate CCS from blastemal and stromal components of WT. Other markers except cyclin D1 do not have a role in the differential diagnosis.
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