Although a variety of ultrastructural and immunohistochemical studies have provided strong support for mesothelial differentiation, their preferential occurence in the genital organs has not yet been well explained. Alternative explanations, including differentiation from muscle, vascular endothelium, and pluripotent mesenchymal cells have also been proposed.
In this study, immunohistochemical phenotypes and the origin of this neoplasm are discussed.
In histologic examination, all adenomatoid tumors were composed of ill-defined, nodular proliferations of variably sized tubules and cysts lined by eosinophilic cuboidal or flattened cells (Figure 1). The cells lining the gland-like structures have small-to-moderate amounts of pale, often vacuolated cytoplasm and small, but conspicuous nucleoli. Nuclear pleomorphism, calcification, and mitotic figures were not seen. All cases contained chronic inflammatory cells either as isolated lymphocytic infiltrates or as true lymphoid follicles (Figure 2).
Immunohistochemically, the neoplastic cells in all tumors were stained strongly positive with pancytokeratin, the mesothelial antigen calretinin and HBME-1 (Figure 3), less intensely with vimentin. EMA and CD31 were consistently negative.
Resim 3: Immunohistochemically, the tumor revealed strong staining for calretinin (Calretinin x100).
The histogenesis of adenomatoid tumors is controversial, and mesonephric, mullerian, endothelial, and mesothelial origins have been suggested[5,12-14]. Previous studies, based on electron microscopic and immunohistochemical findings support a mesothelial origin[1-6].
Since they are almost merely incidental findings in hysterectomy specimens of adult women, the true incidence of these tumors is unknown. Furthermore, they are frequently mistaken macroscopically for leiomyomas, just as in our cases.
Macroscopically, they most often appear as nodular formations with ill- defined margins with the surrounding myometrium, an observation that helps to differentiate the lesion from leiomyomas, which are much more clearly delineated. Only a small proportion of the cases are not identified macroscopically, because they diffusely involve the myometrial wall and lack nodular arrangement. They may be located intramurally and subserosally[1]. The uterine AT in our patient was located intramurally.
There are several histologic types of ATs. Quigley and Hart[15] have described four types: adenoid (also referred as tubular or glandular), angiomatoid, solid, and cystic. Although most tumors feature adenoid or angiomatoid histologic patterns, in our cases all of which represented adenomatoid patterns, and combinations of two or more patterns in each tumor. Tumors are usually solitary, small, and solid, but multiple, multinodular, diffuse, and large cystic lesions have also been described, particularly in the uterus[6]. Cytologic atypia and mitoses are typically absent. In the uterus, they often associated with smooth muscle hypertrophy, whereas extrauterine forms frequently contain intermingled fibroblastic tissue. Some believe that, this is a reactive hyperplasia of indigenous myometrial smooth muscle, and this contention is supported by the absence of smooth muscle in ATs that occur in other sites, such as ovary, mesentery, adrenal glands, and omentum[1]. Others postulate that, this represents a neoplastic component, and the possibility of a collision tumor composed of leiomyoma and adenomatoid tumor cannot be completely disallowed[16,17].
To date, no recurrence or malignant transformation have been reported in ATs. Therefore, the recommended treatment is simple excision of the tumor, if possible.
The histogenesis of AT is controversial. Some authors have suggested, mesonephric, müllerian, endothelial, and mesothelial origin[5,12-14,18]. Their usual subserosal position would suggest an origination from the uterine peritoneum. Alternatively, since most tumors have an intramural location, it has been speculated that the mesothelial component may originate from muscle[1].
It was recently suggested that these tumors originate from mesenchymal stem cells differentiating toward submesothelial or mesothelial cells[18]. However, ultrastrucal and immunohistochemical studies indicates that ATs are benign mesotheliomas. Ultrastructurally, tumor cells showed numerous apical microvilli, abundant cytoplasmic filaments, and desmosomes. Immunohistochemically, the tumor cells are strongly positive for cytokeratin, vimentin, HBME-1 (anti-human mesothelioma antibody), and calretinin but negative for EMA, factor VIII, Ber-EP4, and carcinoembryonic antigen (CEA). These characteristics are all presented in normal mesothelium and in the cells of mesotheliomas.
The reactivity for cytokeratin, calretinin, HBME-1 and vimentin, and consistent negativity for EMA, CD31 in our study suggest a mesothelial origin for adenomatoid tumor, which is compatible with the literature.
A possible explanation of this phenomenon is that ATs arise from inclusions of mesothelium incorporated into subserosal connective tissue or myometrium.
The differential diagnosis is not usually complicated because ATs are easily recognizable. However, macroscopically large cystic tumors may resemble a lymphangioma[19]. Ats with a diffuse, infiltrative growth pattern may cause a diagnostic dilemma. In these instances, because of the unusual setting or pseudoinfiltrative pattern, distinguishing ATs from malignant lesions such as metastatic signet-ring cell adenocarcinoma, epitheloid hemangioendothelioma, germ cell tumor, or sex cord-stromal tumor becomes more important[20,21].
Immunohistochemically, the invariably intense cytoplasmic staining with cytokeratin markers in conjunction with the absence of staining for factor VIII and CD31 in adenomatoid tumors is usually sufficient to exclude vascular tumors and other mesenchymal lesions. Similarly, absence of inhibin expression helps to exclude adrenal cortical or sex cord-stromal neoplasm[1]. Additionaly, ATs may sometimes contain cells with small intracytoplasmic vacuoles and may be confused with signet-ring cell adenocarcinoma. The bland cytologic features and absence of mitoses, histochemical negativity of mucin stains, immunohistochemical carcinoembryonic antigen (CEA) and EMA negativity support the diagnosis of Ats[1,7].
In summary, our immunohistochemical results confirm that ATs of female genital tract have mesothelial origin. Immunohistochemical phenotypes are important in the differential diagnosis. The biologic behaviour of AT is benign and has a good prognosis.
1) Nogales FF, Isaac MA, Hardisson D, Bosincu L, Palacios J, Ordi J, et al. Adenomatoid tumors of the uterus: an analysis of 60 cases. Int J Gynecol Pathol 2002;21:34-40.
2) Klintorp S, Grinsted L, Franzmann MB. Adenomatoid tumor of the uterus. Eur J Obstet Gynecol Reprod Biol 1993;50:255-257.
3) Cheng CL, Wee A. Diffuse uterine adenomatoid tumor in an immunosuppressed renal transplant recipient. Int J Gynecol Pathol 2003;22:198-201.
4) Huang CC, Chang DY, Chen CK, Chou YY, Huang SC. Adenomatoid tumor of the female genital tract. Int J Gynaecol Obstet 1995;50:275-280.
5) Kawamura K, Sekiguchi K, Shibata S, Fukuda J, Kodama H, Tanaka T. Immunohistochemical analysis of adenomatoid tumor of the uterus utilizing of monoclonal antibody HBME-1. Acta Obstet Gynecol Scand 2000;79:798-799.
6) Schwartz EJ, Longacre TA. Adenomatoid tumors of the female and male genital tracts express WT1. Int J Gynecol Pathol 2004;23:123-128.
7) De Rosa G, Boscaino A, Terracciano LM, Giordano G. Giant adenomatoid tumors of the uterus. Int J Gynecol Pathol 1992;11:156-160.
8) Livingston EG, Guis MS, Pearl ML, Stern JL, Brescia RJ. Diffuse adenomatoid tumor of the uterus with a serosal papillary cystic component. Int J Gynecol Pathol
1992;11:288-292.
9) Karayigit E, Mete O, Kilicaslan I, Uysal V. Paratesticular adenomatoid tumor (morphologic and immunohistochemical study of 9 cases). Turkish J Pathol 2006;22:32-36.
10) Travis WD, Lack EE, Azumi N, Tsokos M, Norton J. Adenomatoid tumor of the adrenal gland with ultrastructural and immunohistochemical demonstration of a mesothelial origin. Arch Pathol Lab Med 1990;114: 722-724.
11) Craig JR, Hart WR. Extragenital adenomatoid tumor: evidence for the mesothelial theory of origin. Cancer 1979;43:1678-1681.
12) Mai KT, Yazdi HM, Perkins DG, Isotalo PA. Adenomatoid tumor of the genital tract: evidence of mesenchymal cell origin. Pathol Res Pract 1999;195:605-610.
13) Mazur MT, Kraus FT. Histogenesis of morphologic variations in tumors of the uterine wall. Am J Surg Pathol 1980;4:59-74.
14) Bell DA, Flotte TJ. Factor VIII related antigen in adenomatoid tumors: implications for histogenesis. Cancer 1982;50:932-938.
15) Quigley JC, Hart WR. Adenomatoid tumors of the uterus. Am J Clin Pathol 1981 ;76:627-635.
16) Amre R, Constantino J, Lu S, Charney D. Pathologic quiz case: a 52-year-old woman with a uterine mass. leiomyo-adenomatoid tumor of the uterus. Arch Pathol Lab Med 2005; 129:77-78.
17) Kausch I, Galle J, Buttner H, Bohle A, Jocham D. Leiomyo- adenomatoid tumor of the epididymis. J Urol 2002;168:636.
18) Satoh Y, Hiraguchi M, Matuura T, Takasu K. Immunohistochemical evidence of the mesothelial histogenesis of uterine adenomatoid tumor. J Obstet Gynaecol Res 1998;24:91-101.
19) Iwasaki I, Yu TJ, Tamaru J, Asanuma K. A cystic adenomatoid tumor of the uterus simulating lymphangioma grossly. Acta Pathol Jpn 1985;35:989-993.