Recently published data have suggested a link between active human papillomavirus (HPV) infection and the development of bladder cancer. The aim of our study was to detect HPV DNA in of transitional cell carcinomas of the urinary bladder. We analysed a series of 30 formalin-fixed, paraffin-embedded transitional cell carcinomas of the urinary bladder for the presence of HPV DNA by using in situ hybridization technique. HPV DNA was detected in %3,3 (1/30) of the cases.Our results suggest that HPVDNA may play a minor role in the transitional cell carcinoma development in the urinary bladder.